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Catalog Number Size Price
LS-C287177-10 10 µl $295 
LS-C287177-100 100 µl (1 mg/ml) $514 
TRIM28 / KAP1 Antibody - Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:1000 (1μg/ml). Detection: DAB (left) and green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).
TRIM28 / KAP1 Antibody - Detection of mouse and rat KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TRIM28 / KAP1 Antibody - Detection of human KAP-1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50µg) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TRIM28 / KAP1 Antibody - Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
TRIM28 / KAP1 Antibody - Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TRIM28 / KAP1 Antibody - Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:1000 (1μg/ml). Detection: DAB (left) and green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).
TRIM28 / KAP1 Antibody - Detection of mouse and rat KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
TRIM28 / KAP1 Antibody - Detection of human KAP-1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and HEK293T (50µg) cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TRIM28 / KAP1 Antibody - Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
TRIM28 / KAP1 Antibody - Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 ug) cells. Antibodies: Affinity purified rabbit anti-KAP1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa1‑50, IHC, WB) LS‑C287177

Polyclonal Rabbit anti‑Human TRIM28 / KAP1 Antibody (aa1‑50, IHC, WB) LS‑C287177

Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, ICC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287177-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
TRIM28 / KAP1 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Application:
IHC, ICC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
KAP1 antibody LS-C287177 is an unconjugated rabbit polyclonal antibody to KAP1 (TRIM28) (aa1-50) from human. It is reactive with human and mouse. Validated for ICC, IHC, IP and WB. Cited in 1 publication.
Target
Human TRIM28 / KAP1
Synonyms
TRIM28 | E3 SUMO-protein ligase TRIM28 | KRIP-1 | Nuclear corepressor KAP-1 | KAP-1 | KAP1 | KRAB-interacting protein 1 | TIF1B | RNF96 | TIF1-beta | TIF1beta | Tripartite motif-containing 28 | KRAB-associated protein 1 | RING finger protein 96 | TF1B | Tif1beta zinc finger protein | Tripartite motif containing 28
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 10155 corresponding to amino acids between 1 and 50 of UniprotID: Q13263
Epitope
aa1-50
Specificity
Region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).
Applications
  • IHC (1:500 - 1:2000)
  • ICC (1:500 - 1:2500)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRIM28 / KAP1
Q13263 NM_005762 NP_005753.1

LSBio Ratings

TRIM28 / KAP1 Antibody (aa1-50) for IHC, ICC, WB/Western, IP LS-C287177 has an LSBio Rating of
Publications (4)

Publications (1)

Absolute Quantification of Transcription Factors Reveals Principles of Gene Regulation in Erythropoiesis. Mark A Gillespie, Carmen G Palii, Daniel Sanchez-Taltavull, Paul Shannon, William J R Longabaugh, Damien J Downes, Karthi Sivaraman, Herbert M Espinoza, Jim R Hughes, Nathan D Price, Theodore J Perkins, Jeffrey A Ranish, Marjorie Brand. Molecular cell. 2020 June;78:960-974.e11.

Customer Reviews (0)


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Requested From: United States
Date Requested: 12/15/2024