Human TP73 / p73

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

5B429

Unconjugated

Protein G purified

Azide-free

This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-Terminus of human p73. It does not cross react with p53.

N-Terminus

Human TP73 / p73. P73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

• IHC
• IHC - Paraffin (5 - 15 µg/ml)
• IHC - Frozen (5 - 15 µg/ml)
• ICC (1:10 - 1:500)
• Immunofluorescence (1:10 - 1:500)
• Western blot (2 µg/ml)
• Immunoprecipitation (1:10 - 1:500)
• Chromatin Immunoprecipitation (1:10 - 1:500)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS. No stabilizing proteins added.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15350 NCBI: NM_005427 NP_005418.1