Human TP73 / p73

Mouse

Human, Monkey (tested or 100% immunogen sequence identity)

Mouse (at least 90% immunogen sequence identity)

IgG1 Monoclonal

38C674.2

Unconjugated

Protein G purified

Unmodified

This antibody was developed against a peptide corresponding to amino acid residues 2-13 LYVGDPARHLAT of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine. Percent identity by BLAST analysis: Human, Gibbon, Monkey (100%); Mouse (92%).

aa2-13

A peptide corresponding to amino acid residues 2-13 LYVGDPARHLAT of human dNp73. Mouse and human sequences are more than 95 % identical at these amino acid residues. In mouse, alanine at position 8 is replaced by methionine.

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1 - 3 µg/ml)
• Chromatin Immunoprecipitation

Immunohistochemistry: LS-B2085 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2085 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide, 0.05% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15350 NCBI: NM_005427 NP_005418.1