Human TP73 / p73

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

5B1288

Unconjugated

Protein G purified

Azide-free

This antibody was raised against full-length human p73. The epitope is thought to lie around the center of the molecule (NP_005418).

Human TP73 / p73. It reacts with alpha, beta, gamma and delta isoforms of mouse and human p73 as well as the dominant negative p73 (Costanzo, et al, 2002). Because of its reactivity pattern it may be regarded as anti-pan p73. Immunoprecipitation details can be found in Sayan et al (2005). NB100-56674 has been shown to recongize all the known alternative splicing variants of human and mouse p73 (Costanzo et al, 2002). The antibody does not cross react with p53.

• ICC (1:10 - 1:2000)
• Immunofluorescence (1:10 - 1:2000)
• Western blot (1 - 3 µg/ml)
• Immunoprecipitation (1:10 - 1:500)
• Chromatin Immunoprecipitation (1:10 - 1:500)

Reported use for Chromatin Immunoprecipitation assay (See accardi et al). Immunocytochemistry/Immunofluorescence and Immunoprecipitation (see Sayan et al) The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

PBS. No stabilizing proteins added.

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15350 NCBI: NM_005427 NP_005418.1