Human TNFRSF9 / 4-1BB / CD137

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

4B4-1

Unconjugated

Protein A purified

Fab'2. Also available Unmodified or Azide-free.

Mouse IgG1, kappa.

Antibody 4B4-1 recognizes an extracellular epiopte of the human CD137 molecule of about 35 kd on activated T cells.

• Flow Cytometry
• ELISA

Ficoll prepared human peripheral blood lymphocytes were stimulated overnight in culture with 10 ng/ml PMA and 1mM Ionomicin. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD137 F(ab')2 at 5 ug/ml. Cells were washed twice and incubated with 2o reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.75 log10 fluorescent units when compared to a Mouse IgG1 F(ab')2 negative control. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Sterile Solution 50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, No preservatives added

Store at 4°C. Do not freeze. Open under aseptic conditions.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q07011 NCBI: NM_001561 NP_001552.2