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Antibodies > TNFRSF11A / RANK > LS-C662057
 
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Catalog Number Size Price
LS-C662057-10 10 µg $318 
LS-C662057-100 100 µg $470 
TNFRSF11A / RANK Antibody - Immunohistochemistry - Anti-RANK Picoband Antibody
TNFRSF11A / RANK Antibody - Immunohistochemistry - Anti-RANK Picoband Antibody
TNFRSF11A / RANK Antibody - Western blot analysis of RANK using anti-RANK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: HEPG2 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD.
TNFRSF11A / RANK Antibody - Western blot - Anti-RANK Picoband Antibody
TNFRSF11A / RANK Antibody - Immunohistochemistry - Anti-RANK Picoband Antibody
TNFRSF11A / RANK Antibody - Immunohistochemistry - Anti-RANK Picoband Antibody
TNFRSF11A / RANK Antibody - Western blot analysis of RANK using anti-RANK antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: HEPG2 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANK antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANK at approximately 80KD. The expected band size for RANK is at 66KD.
TNFRSF11A / RANK Antibody - Western blot - Anti-RANK Picoband Antibody
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Polyclonal Rabbit anti‑Human TNFRSF11A / RANK Antibody (IHC, WB) LS‑C662057

Polyclonal Rabbit anti‑Human TNFRSF11A / RANK Antibody (IHC, WB) LS‑C662057

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Antibody:
TNFRSF11A / RANK Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662057-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TNFRSF11A / RANK Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
RANK antibody LS-C662057 is an unconjugated rabbit polyclonal antibody to human RANK (TNFRSF11A). Validated for IHC and WB.
Target
Human TNFRSF11A / RANK
Synonyms
TNFRSF11A | CD265 | CD265 antigen | ODFR | LOH18CR1 | OPTB7 | OSTS | PDB2 | RANK | Receptor activator of NF-KB | TRANCER | FEO | OFE
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human RANK (235-262 aa YRKKGKALTANLWHWINEACGRLSGDKE), different from the related mouse sequence by seven amino acids.
Specificity
Ubiquitous expression with high levels in skeletal muscle, thymus, liver, colon, small intestine and adrenal gland.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFRSF11A / RANK
Q9Y6Q6 NM_003839 NP_003830.1

Publications (0)

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Applications: IHC, IHC - Paraffin, Western blot
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IHC‑plus™ TNFRSF11A / RANK Antibody (aa326‑616, clone 9A725) LS‑B2076
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See all TNFRSF11A / RANK Antibodies

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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