Target
Dog TJP2 / ZO2 / ZO-2
Synonyms
TJP2 | C9DUPq21.11 | DUP9q21.11 | DFNA51 | ZO-2 | Zona occludens 2 | Zonula occludens protein 2 | Tight junction protein 2 | Tight junction protein ZO-2 | ZO2 | X104 | Zona occludens protein 2
Reactivity
Dog, Human, Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide derived from the central portion of the ZO-2 protein.
Specificity
Specific for the canine ZO-2 protein (~160kD). Does exhibit cross-reactivity with a few proteins of unknown identity in the 50-85kD region. Does not appear to cross-react with the related ZO-1 protein. Species cross-reactivity: human and mouse.
Applications
- IHC
- Western blot (1 - 4 µg/ml)
- ELISA (0.1 - 1 µg/ml)
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Usage
Suitable for use in ELISA, Immunohistochemistry and Western Blot. ELISA: 0.1-1 ug/ml. Western Blot: 1-4 ug/ml. Immunohistochemistry (Mouse liver tissues): 4-5 ug/ml. Note: Weak fixation of tissues with 2% paraformaldehyde/picric acid followed by NO post-fixation is required to produce staining. Positive control: HeLa and canine MDCK cell lysates and mouse liver homogenates.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee