Human THBD / CD141 / Thrombomodulin

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Recombinant Monoclonal

rTHBD/1591

Unconjugated

Protein A/G purified

Azide-free, Carrier-free. Also available Unmodified.

Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein. Antigen Molecular Weight: ~100 kDa

aa 69-194

It recognizes a protein of 75 kDa , identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.

• IHC - Paraffin (1 - 2 µg/ml)
• ELISA (2 - 4 µg/ml)

ELISA (Use Ab at 2-4 ug/ml for coating) - Immunohistology (Formalin-fixed - 1-2 ug/ml for 30 minutes at RT - Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.

10 mM PBS

Store at -20°C to -80°C for up to 2 years.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P07204 NCBI: NM_000361 NP_000352.1