Target
Mouse THBD / CD141 / Thrombomodulin
Synonyms
THBD | BDCA3 | CD141 antigen | CD141 | AHUS6 | Fetomodulin | THPH12 | TM | THRM | Thrombomodulin
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Purification
Affinity purified
Immunogen
NS0-derived rmThrombomodulin extracellular domain.
Epitope
Extracellular Domain
Specificity
Selected for use as a detection antibody in mouse Thrombomodulin Western Blots.
Applications
- Western blot (0.1 - 0.2 µg/ml)
- Flow Cytometry (50 µg/ml)
Usage
Suitable for use in Flow Cytometry and Western Blot. Flow Cytometry: 50 ug/ml; 10 ul labels 1-2.5x10^5 cells in a total reaction volume 200ul. Tested on bEnd.3 cells. Western Blot: 0.1-0.2 ug/ml; The detection limit is ~0.5 ng/lane and 25 ng/lane under non-reducing and reducing conditions, respectively. Shows less than 1% cross-reactivity with recombinant human Thrombomodulin. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Reconstitution
TBS, pH7.3, containing 0.1% BSA
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About THBD / CD141 / Thrombomodulin