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Catalog Number Size Price
LS-C358873-30 30 µl (1 mg/ml) $255 
LS-C358873-100 100 µl (1 mg/ml) $299 
LS-C358873-200 200 µl (1 mg/ml) $351 
SYT1 / Synaptotagmin Antibody - Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SYT1 / Synaptotagmin Antibody - Immunofluorescent analysis of Synaptotagmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SYT1 / Synaptotagmin Antibody - Western blot analysis of Synaptotagmin expression in HEK293T (A); mouse brain (B) whole cell lysates.
SYT1 / Synaptotagmin Antibody - Immunohistochemical analysis of Synaptotagmin staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SYT1 / Synaptotagmin Antibody - Immunofluorescent analysis of Synaptotagmin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
SYT1 / Synaptotagmin Antibody - Western blot analysis of Synaptotagmin expression in HEK293T (A); mouse brain (B) whole cell lysates.
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Polyclonal Rabbit anti‑Human SYT1 / Synaptotagmin Antibody (Internal, IHC, IF, WB) LS‑C358873

Polyclonal Rabbit anti‑Human SYT1 / Synaptotagmin Antibody (Internal, IHC, IF, WB) LS‑C358873

Antibody:
SYT1 / Synaptotagmin Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C358873-30
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SYT1 / Synaptotagmin Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Synaptotagmin antibody LS-C358873 is an unconjugated rabbit polyclonal antibody to Synaptotagmin (SYT1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC and WB.
Target
Human SYT1 / Synaptotagmin
Synonyms
SYT1 | SYT | SVP65 | Synaptotagmin | Synaptotagmin 1 | SytI | Synaptotagmin I | Synaptotagmin-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human Synaptotagmin.
Epitope
Internal
Specificity
Recognizes endogenous levels of Synaptotagmin protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SYT1 / Synaptotagmin
P21579 NM_005639 NP_005630.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024