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| Catalog Number | Size | Price |
|---|---|---|
| LS-B5899-50 | 50 µg (0.5 mg/ml) | $485 |
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2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human SYT1 / Synaptotagmin Antibody (aa261‑273, IHC, WB) LS‑B5899
IHC‑plus™ Polyclonal Goat anti‑Human SYT1 / Synaptotagmin Antibody (aa261‑273, IHC, WB) LS‑B5899
Note: This antibody replaces LS-C112808
Antibody:
SYT1 / Synaptotagmin Goat anti-Human Polyclonal (aa261-273) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SYT1 / Synaptotagmin Goat anti-Human Polyclonal (aa261-273) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish
Format:
Unconjugated, Unmodified
Specifications
Description
Synaptotagmin antibody LS-B5899 is an unconjugated goat polyclonal antibody to Synaptotagmin (SYT1) (aa261-273) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human SYT1 / Synaptotagmin
Synonyms
SYT1 | SYT | SVP65 | Synaptotagmin | Synaptotagmin 1 | SytI | Synaptotagmin I | Synaptotagmin-1
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-RDLQSAEKEEQEK, from the internal region of the protein sequence according to NP_005630.1.
Epitope
aa261-273
Specificity
Human SYT1 / Synaptotagmin. Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.
Applications
- IHC
- IHC - Paraffin (3.75 µg/ml)
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:2000. Western blot: Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720). Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SYT1 / Synaptotagmin
Validation
Anti-SYT1 / Synaptotagmin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-SYT1 / Synaptotagmin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ SYT1 / Synaptotagmin Antibody (aa261-273) for IHC, WB/Western LS-B5899 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Yu Liu , Miao Yu , Xuanze Shang, My Hong Hoai Nguyen, Shanmuganathan Balakrishnan, Rachel Sager, Huaiyu Hu. Scientific reports. 2020 May;10:7795.
Customer Reviews (0)
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/27/2024
Date Requested: 11/27/2024
