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Catalog Number	Size	Price
LS-C662751-10	10 µg	$318
LS-C662751-100	100 µg	$470

SYT1 / Synaptotagmin Antibody - Immunohistochemistry - Anti-Synaptotagmin 1 Picoband antibody

SYT1 / Synaptotagmin Antibody - Western blot analysis of Synaptotagmin 1 using anti-Synaptotagmin 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Synaptotagmin 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Synaptotagmin 1 at approximately 65KD. The expected band size for Synaptotagmin 1 is at 47KD.

SYT1 / Synaptotagmin Antibody - Western blot - Anti-Synaptotagmin 1 Picoband antibody

SYT1 / Synaptotagmin Antibody - Flow Cytometry analysis of LLC cells using anti-Synaptotagmin 1 antibody. Overlay histogram showing LLC cells stained with anti-Synaptotagmin 1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

SYT1 / Synaptotagmin Antibody - Flow Cytometry analysis of PC-3 cells using anti-Synaptotagmin 1 antibody. Overlay histogram showing PC-3 cells stained with anti-Synaptotagmin 1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

SYT1 / Synaptotagmin Antibody - Flow Cytometry analysis of A549 cells using anti-Synaptotagmin 1 antibody. Overlay histogram showing A549 cells stained with anti-Synaptotagmin 1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Synaptotagmin 1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

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Polyclonal Rabbit anti‑Human SYT1 / Synaptotagmin Antibody (IHC, WB) LS‑C662751

Name: Anti-SYT1 / Synaptotagmin Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662751-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

SYT1 / Synaptotagmin Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662751-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SYT1 / Synaptotagmin Rabbit anti-Human Polyclonal Antibody

Application:

IHC, IHC-P, IHC-Fr, ICC, WB, Flo

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

Synaptotagmin antibody LS-C662751 is an unconjugated rabbit polyclonal antibody to human Synaptotagmin (SYT1). Validated for Flow, ICC, IHC and WB.

Target

Human SYT1 / Synaptotagmin

Synonyms

See All SYT1 / Synaptotagmin Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence of human Synaptotagmin 1 (RRPIAQWHTLQVEEEVDAMLAVKK).

Specificity

Expressed in melanocytes (PubMed:23999003).

Applications

IHC
IHC - Paraffin
IHC - Frozen
ICC
Western blot
Flow Cytometry

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SYT1 / Synaptotagmin

Uniprot: P21579 NCBI: NM_005639 NP_005630.1