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Antibodies > SREBF1 / SREBP-1 > LS-C801082
 
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Catalog Number Size Price
LS-C801082-100 100 µl (1 mg/ml) $379 
LS-C801082-200 200 µl (1 mg/ml) $421 
SREBF1 / SREBP-1 Antibody - 1/100 staining human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SREBF1 / SREBP-1 Antibody - 1/100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - Western blot analysis of SREBP-1 phosphorylation expression in TNF treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
SREBF1 / SREBP-1 Antibody - 1/100 staining human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - 1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SREBF1 / SREBP-1 Antibody - 1/100 staining human brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SREBF1 / SREBP-1 Antibody - Western blot analysis of SREBP-1 phosphorylation expression in TNF treated Jurkat whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human SREBF1 / SREBP‑1 Antibody (phospho‑Ser439, IHC, IF, WB) LS‑C801082

Polyclonal Rabbit anti‑Human SREBF1 / SREBP‑1 Antibody (phospho‑Ser439, IHC, IF, WB) LS‑C801082

Submit Review LSBio Guarantee
Antibody:
SREBF1 / SREBP-1 Rabbit anti-Human Polyclonal (pSer439) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801082-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SREBF1 / SREBP-1 Rabbit anti-Human Polyclonal (pSer439) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SREBP-1 antibody LS-C801082 is an unconjugated rabbit polyclonal antibody to SREBP-1 (SREBF1) (pSer439) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human SREBF1 / SREBP-1
Synonyms
SREBF1 | BHLHd1 | SREBP-1 | SREBP-1c | SREBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human SREBP-1 around the phosphorylation site of Serine 439.
Epitope
pSer439
Specificity
Phospho-SREBP-1 (Ser439) Antibody detects endogenous levels of SREBP-1 only when phosphorylated at Serine 439.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SREBF1 / SREBP-1
P36956 NM_004176 NP_004167.3

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IHC‑plus™ SREBF1 / SREBP‑1 Antibody (aa300‑400) LS‑B93
Species: Human, Mouse, Rat, Bovine, Goat, Pig, Sheep
Applications: IHC, IHC - Paraffin, Western blot
SREBF1 / SREBP-1 Antibody - SREBP1 Antibody (2A4) - Western blot of SREBP1 expression in HeLa lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SREBF1 / SREBP‑1 Antibody (aa301‑407, clone 2A4) LS‑C143035
Species: Human, Mouse, Rat, Hamster
Applications: Western blot
SREBF1 / SREBP-1 Antibody - Immunohistochemical analysis of SREBP1 (pS439) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SREBF1 / SREBP‑1 Antibody (phospho‑Ser439) LS‑C368524
Species: Human, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, Western blot
SREBF1 / SREBP-1 Antibody - Western Blot analysis of 1) mouse liver, 2) HeLa, 3) mouse brain cells using primary antibody diluted at 1:1000 (4°C overnight) . Secondary antibody: Goat Anti-rabbit IgG IRDye 800 (diluted at 1:5000, 25°C, 1 hour).
SREBF1 / SREBP‑1 Antibody LS‑C813280
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
SREBF1 / SREBP-1 Antibody - Anti-SREBF1 / SREBP-1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SREBF1 / SREBP‑1 Antibody (aa461‑510) LS‑B6968
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
SREBF1 / SREBP-1 Antibody - SP2/0 Cell Lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SREBF1 / SREBP‑1 Antibody (aa28‑77) LS‑C30185
Species: Mouse
Applications: Western blot
See all SREBF1 / SREBP-1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/15/2024
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