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Catalog Number Size Price
LS-C9413-100 100 µl $634 
Antibody

Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (IHC, WB) LS‑C9413

Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (IHC, WB) LS‑C9413

Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$634
LS-C9413-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Osteopontin antibody LS-C9413 is an unconjugated rabbit polyclonal antibody to human Osteopontin (SPP1). Validated for ELISA, IHC and WB.
Target
Human SPP1 / Osteopontin
Synonyms
SPP1 | BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
A synthetic peptide C-KSKKFRRPDIQYPD corresponding to human osteopontin proteins conjugated to KLH using maleimide. A residue of cysteine was added to the N-Terminus end to facilitate coupling. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%); Marmoset (93%).
Specificity
Recognizes the full length Human osteopontin protein at 66kD, C-terminal fragments of both thrombin and MMP-cleaved OPN at 32kD. Does not recognizes the 40kD N-terminal fragment.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:250 - 1:1000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 1:10000-1:50000. Immunohistochemistry (paraffin): 1:50-1:150. Western Blot: 1:250-1:1000.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide, 40% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024