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SPP1 / Osteopontin
secreted phosphoprotein 1
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
| Gene Name: | secreted phosphoprotein 1 |
| Synonyms: | SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I |
| Target Sequences: | NM_000582 NP_000573.1 P10451 |
Publications (8)
1
Mapping of functional epitopes of osteopontin by monoclonal antibodies raised against defined internal sequences. Kon S, Yokosaki Y, Maeda M, Segawa T, Horikoshi Y, Tsukagoshi H, Rashid MM, Morimoto J, Inobe M, Shijubo N, Chambers AF, Uede T. Journal of cellular biochemistry. 2002 84:420-32.
[PubMed:11787071]
2
Osteopontin induces beta-catenin signaling through activation of Akt in prostate cancer cells. Robertson BW, Chellaiah MA. Experimental cell research. 2010 316:11-Jan.
[PubMed:19850036]
[PMC:PMC2787770]
Related Antibodies: LS-B425.
3
Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy. Rui YF, Lui PP, Rolf CG, Wong YM, Lee YW, Chan KM. Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA. 2012 20:1409-17. (IHC; Human)
[PubMed:21946950]
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Antibodies
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(7)
Primary
(137)
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(25)
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(38)
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(5)
Target
SPP1 / Osteopontin
(222)
Reactivity
Human
(135)
Mouse
(49)
Rat
(41)
Bovine
(3)
Canine
(1)
Chicken
(2)
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(14)
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(6)
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(31)
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(93)
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(35)
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(100)
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53
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(32)
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(68)
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230-310 aa, C-terminal
(1)
Amino Acid: 167-314
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Leu285
(1)
aa164-179
(1)
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(1)
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(1)
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(1)
aa28-47
(1)
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(1)
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(1)
aa59-74
(1)
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(1)
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(218)
Yes
(4)
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(1)
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(1)
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(21)
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(23)
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(2)
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(2)
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(3)
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(6)
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(13)
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(3)
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(1)
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(1)
Wheat Germ Extract
(3)
Yeast
(1)
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Purified
(2)
Low Endotoxin Level
Low endotoxin level
(6)
Biologically Active
Biologically active
(1)
Cancer
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Mouse, Dog, Rat, Pig, Human
ELISA, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$460
SPP1 / Osteopontin Mouse anti-Human Monoclonal (C-Terminus) (1B20) Antibody
Rabbit, Rat, Human
ICC, IHC, IHC-P, IP, WB
Unconjugated
0.1 ml/$460
Cancer
SPP1 / Osteopontin Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$545
SPP1 / Osteopontin Goat anti-Human Polyclonal (aa288-299) Antibody
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$397
SPP1 / Osteopontin Goat anti-Mouse Polyclonal (PE) Antibody
Mouse
Flo
PE Conjugated
100 Tests/$811
SPP1 / Osteopontin Mouse anti-Human Monoclonal (X1) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa14-40) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
200 µl/$734
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa273-301) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
HEK 293 Cells
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
60.0-65.0 kDa
10 µg/$268; 50 µg/$349
Sandwich
96-Well Strip Plate
Bovine
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$889
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$585
Sandwich CLIA
96-Well Strip Plate
Mouse
6.86 - 5000 pg/ml
Chemiluminescent
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$871
Sandwich
96-Well Microplate
Pig
62.5 - 4000 pg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$728
Sandwich
96-Well Strip Plate
Human
62.5 - 4000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum, Urine
1 Plate/$582
Cancer
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa14-40) Antibody
Human
IF, IHC, IHC-P, WB
Unconjugated
100 µl/$460
SPP1 / Osteopontin Mouse anti-Human Monoclonal (7C5H12) Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µl/$459
Sandwich
96-Well Microplate
Human
50 - 18000 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum
1 Plate/$534
Sandwich
96-Well Strip Plate
Mouse
39 - 2500 pg/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$610
Sandwich
96-Well Microplate
Rat
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$586
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa261-310) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100 µl/$515
SPP1 / Osteopontin Mouse anti-Human Monoclonal (aa17-314) Antibody
Human
ELISA, WB
Unconjugated
100 µl/$445
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
SPP1 / Osteopontin Rabbit anti-Mouse Polyclonal (FITC) Antibody
Mouse
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa17-302) (Biotin) Antibody
Human
ELISA
Biotin Conjugated
50 µg/$294; 100 µg/$360
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