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Catalog Number Size Price
LS-C408135-10 10 µg $318 
LS-C408135-100 100 µg $470 
SPP1 / Osteopontin Antibody - Western blot analysis of OPN using anti-OPN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of eachlane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulosemembrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN antibody at 0.5ug/ml 4? overnight, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for OPN at approximately 66KD (OPN precursor) and 40KD (OPN cleaved).
SPP1 / Osteopontin Antibody - Osteopontin antibody Western blot. All lanes: Anti Osteopontin at 0.5 ug/ml. Lane 1: Mouse Pancreas Tissue Lysate at 50 ug. Lane 2: JURKAT Whole Cell Lysate at 40 ug. Lane 3: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 66 kD. Observed band size: 66 kD.
SPP1 / Osteopontin Antibody - Western blot analysis of OPN using anti-OPN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of eachlane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulosemembrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPN antibody at 0.5ug/ml 4? overnight, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. Specific bands were detected for OPN at approximately 66KD (OPN precursor) and 40KD (OPN cleaved).
SPP1 / Osteopontin Antibody - Osteopontin antibody Western blot. All lanes: Anti Osteopontin at 0.5 ug/ml. Lane 1: Mouse Pancreas Tissue Lysate at 50 ug. Lane 2: JURKAT Whole Cell Lysate at 40 ug. Lane 3: HEPG2 Whole Cell Lysate at 40 ug. Predicted band size: 66 kD. Observed band size: 66 kD.
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Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (aa281‑314, WB) LS‑C408135

Polyclonal Rabbit anti‑Human SPP1 / Osteopontin Antibody (aa281‑314, WB) LS‑C408135

Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa281-314) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C408135-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SPP1 / Osteopontin Rabbit anti-Human Polyclonal (aa281-314) Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Osteopontin antibody LS-C408135 is an unconjugated rabbit polyclonal antibody to Osteopontin (SPP1) (aa281-314) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human SPP1 / Osteopontin
Synonyms
SPP1 | BSPI | BNSP | Bone sialoprotein 1 | ETA-1 | Nephropontin | OPN | Secreted phosphoprotein 1 | SPP-1 | SPP1/CALPHA1 fusion | Urinary stone protein | Uropontin | Osteopontin | Bone sialoprotein I
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Osteopontin (281-314 aa HEDMLVVDPKSKEEDKHLKFRISHELDSASSEVN), different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.
Epitope
aa281-314
Specificity
Bone. Found in plasma.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SPP1 / Osteopontin
P10451 NM_000582 NP_000573.1

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Requested From: United States
Date Requested: 11/24/2024