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Catalog Number Size Price
LS-C407831-10 10 µg $318 
LS-C407831-100 100 µg $470 
SOX4 Antibody - Western blot analysis of SOX4 using anti-SOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
SOX4 Antibody - SOX4 antibody Western blot. All lanes: Anti SOX4 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: 293T Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.
SOX4 Antibody - Western blot analysis of SOX4 using anti-SOX4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX4 at approximately 47KD. The expected band size for SOX4 is at 47KD.
SOX4 Antibody - SOX4 antibody Western blot. All lanes: Anti SOX4 at 0.5 ug/ml. Lane 1: MCF-7 Whole Cell Lysate at 40 ug. Lane 2: 293T Whole Cell Lysate at 40 ug. Predicted band size: 47 kD. Observed band size: 47 kD.
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Polyclonal Rabbit anti‑Human SOX4 Antibody (aa1‑31, WB) LS‑C407831

Polyclonal Rabbit anti‑Human SOX4 Antibody (aa1‑31, WB) LS‑C407831

Antibody:
SOX4 Rabbit anti-Human Polyclonal (aa1-31) Antibody
Application:
WB
Reactivity:
Human, Mouse, Bovine, Dog, Guinea pig, Pig, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407831-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SOX4 Rabbit anti-Human Polyclonal (aa1-31) Antibody
Application:
WB
Reactivity:
Human, Mouse, Bovine, Dog, Guinea pig, Pig, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
SOX4 antibody LS-C407831 is an unconjugated rabbit polyclonal antibody to SOX4 (aa1-31) from human. It is reactive with human, mouse, bovine and other species. Validated for WB.
Target
Human SOX4
Synonyms
SOX4 | EVI16 | SRY-related HMG-box gene 4 | Transcription factor SOX-4
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Dog, Guinea pig, Pig, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human SOX4 (1-31 aa MVQQTNNAENTEALLAGESSDSGAGLELGIA), identical to the related mouse sequence.
Epitope
aa1-31
Specificity
Testis, brain, and heart.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SOX4
Q06945 NM_003107 NP_003098.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 2/1/2025