Target
Human SOX2
Synonyms
SOX2 | MCOPS3 | Transcription factor SOX2 | ANOP3 | Transcription factor SOX-2 | SRY-related HMG-box gene 2
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence YNMMETELKPPGPQQ-C, from the N-Terminus of protein sequence according to NP_003097.1.
Applications
- Immunofluorescence (10 µg/ml)
- Western blot (0.1 - 0.3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 37kDa band observed in nuclear lysates of cell line MCF7 and in lysates of Human glioblastoma cell line U251 (calculated MW of 34.3kDa according to )NP_003097.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nuclei of U251 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee