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| Catalog Number | Size | Price |
|---|---|---|
| LS-C210330-500 | 500 µl | $539 |
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Monoclonal Rabbit anti‑Human SOX2 Antibody (clone SP76, N‑Terminus, IHC, WB) LS‑C210330
Monoclonal Rabbit anti‑Human SOX2 Antibody (clone SP76, N‑Terminus, IHC, WB) LS‑C210330
Antibody:
SOX2 Rabbit anti-Human Monoclonal (N-Terminus) (SP76) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
SOX2 Rabbit anti-Human Monoclonal (N-Terminus) (SP76) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
SOX2 antibody LS-C210330 is an unconjugated rabbit monoclonal antibody to human SOX2 (N-Terminus). Validated for IHC and WB.
Target
Human SOX2
Synonyms
SOX2 | MCOPS3 | Transcription factor SOX2 | ANOP3 | Transcription factor SOX-2 | SRY-related HMG-box gene 2
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Predicted
Human, Bovine, Dog, Frog (at least 90% immunogen sequence identity)
Clonality
IgG
Monoclonal
Clone
SP76
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to N-Terminus of human SOX-2 protein.
Epitope
N-Terminus
Specificity
Human SOX2
Applications
- IHC
- IHC - Paraffin (1:100)
- Western blot (1:25)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
IMMUNOHISTOCHEMISTRY: Formalin-fixed, paraffin-embedded tissues are suitable for use with this primary antibody. Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols. Antibody Dilution: If using the concentrate format of this product, dilute the antibody 1:100. Antigen Retrieval: Boil tissue section in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 30 minutes at room temperature. Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween. IHC Positive control: Newborn brain. WESTERN BLOT: Recommended starting protocol: Dilute the antibody 1:25. Incubate for 1 hour at room temperature. The dilution is an estimate. actual results may differ because of variability in methods and protocols. Positive Control: MCF7 cell lysate.
Presentation
10 mM PBS, pH 7.4, 0.1% Sodium Azide, BSA
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About SOX2
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Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Frog
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/19/2024
Date Requested: 11/19/2024
