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Catalog Number	Size	Price
LS-C756654-10	10 µg	$318
LS-C756654-100	100 µg	$470

SMC3 / HCAP Antibody - IHC analysis of SMC3 using anti-SMC3 antibody. SMC3 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-SMC3 antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

SMC3 / HCAP Antibody - IHC analysis of SMC3 using anti-SMC3 antibody. SMC3 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml mouse anti-SMC3 antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

SMC3 / HCAP Antibody - Western blot analysis of SMC3 using anti-SMC3 antibody. Electrophoresis was performed on a 8% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human A549 whole cell lysate,Lane 3: human MCF-7 whole cell lysate,Lane 4: human MDA-MB-453 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SMC3 antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

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Monoclonal Mouse anti‑Human SMC3 / HCAP Antibody (IHC, WB) LS‑C756654

Name: Anti-SMC3 / HCAP Antibody | Mouse anti-Human Monoclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C756654-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

SMC3 / HCAP Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$318

LS-C756654-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SMC3 / HCAP Mouse anti-Human Monoclonal Antibody

Application:

IHC-P, WB

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

HCAP antibody LS-C756654 is an unconjugated mouse monoclonal antibody to HCAP (SMC3) from human. It is reactive with human, mouse and rat. Validated for IHC and WB.

Target

Human SMC3 / HCAP

Synonyms

See All SMC3 / HCAP Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG1 Monoclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the C-Terminus of human SMC3(1178-1216 aa ELLESADKFYGVKFRNKVSHIDVITAEMAKDFVEDDTTH), identical to the related mouse sequence.

Specificity

No cross reactivity with other proteins.

Applications

IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Applications should be user optimized.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.