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Catalog Number	Size	Price
LS-C407649-10	10 µg	$318
LS-C407649-100	100 µg	$470

SLC6A4 / SERT Antibody - SLC6A4 antibody IHC-paraffin. IHC(P): Human Mammary Cancer Tissue.

SLC6A4 / SERT Antibody - SLC6A4 antibody IHC-paraffin. IHC(P): Rat Brain Tissue.

SLC6A4 / SERT Antibody - Western blot analysis of SLC6A4 using anti-SLC6A4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human COLO-320 whole cell lysates. Lane 5: human K562 whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: rat heart tissue lysates, Lane 9: mouse heart tissue lysates, Lane 10: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC6A4 at approximately 90KD. The expected band size for SLC6A4 is at 70KD.

SLC6A4 / SERT Antibody - SLC6A4 antibody Western blot. All lanes: Anti SLC6A4 at 0.5 ug/ml. Lane 1: A549 Whole Cell Lysate at 40 ug. Lane 2: MM231 Whole Cell Lysate at 40 ug. Lane 3: HELA Whole Cell Lysate at 40 ug. Lane 4: 22RV1 Whole Cell Lysate at 40 ug. Predicted band size: 70 kD. Observed band size: 95 kD.

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Polyclonal Rabbit anti‑Human SLC6A4 / SERT Antibody (aa57‑85, IHC, WB) LS‑C407649

Name: Anti-SLC6A4 / SERT Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C407649-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

SLC6A4 / SERT Rabbit anti-Human Polyclonal (aa57-85) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

$318

LS-C407649-10

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SLC6A4 / SERT Rabbit anti-Human Polyclonal (aa57-85) Antibody

Application:

IHC, IHC-P, WB

Reactivity:

Human, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

SERT antibody LS-C407649 is an unconjugated rabbit polyclonal antibody to SERT (SLC6A4) (aa57-85) from human. It is reactive with human and rat. Validated for IHC and WB.

Target

Human SLC6A4 / SERT

Synonyms

See All SLC6A4 / SERT Antibodies

Host

Rabbit

Reactivity

Human, Rat (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

A synthetic peptide corresponding to a sequence at the N-Terminus of human SLC6A4 (57-85 aa DDTRHSIPATTTTLVAELHQGERETWGKK), different from the related mouse and rat sequences by six amino acids.

Epitope

aa57-85

Specificity

Expressed in platelets (at protein level). .

Applications

IHC
IHC - Paraffin (0.5 - 1 µg/ml)
Western blot (0.1 - 0.5 µg/ml)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

WB: The detection limit for SLC6A4 is approximately 0.1 ng/lane under reducing conditions. IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.

Presentation

Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SLC6A4 / SERT

Uniprot: P31645 NCBI: NM_001045 NP_001036.1