Target
Mouse SLC2A1 / GLUT-1
Synonyms
SLC2A1 | EIG12 | GLUT1DS | DYT17 | DYT18 | DYT9 | Glucose transporter 1 | HepG2 glucose transporter | Glucose transporter GLUT1 | GLUT-1 | GLUT1 | PED
Reactivity
Mouse, Human, Rat, Bovine, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
A 12 AA peptide sequence near the C-Terminus of mouse/rat brain glucose transporter-1 (Glut-1). A cysteine has been added at the NH2 terminus for coupling to KLH.
Specificity
Detects Glut-1. No significant sequence homology with other Glut proteins. The Species cross-reactivity: 100% conserved in human, mouse, rat, rabbit, bovine and porcine and 90% conserved in chicken (9/12 aa).
Applications
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:50000)
Usage
Suitable for use in ELISA and Western Blot. Western Blot: 1:1000-1:5000. Detects a major band ~42-45kD using Glut-1 positive control from purified rat brain membranes. Not recommended to heat the samples prior to loading to prevent Glut protein aggregation. ELISA: 1:10000-1:50000. Control peptide can be used to coat ELISA plates at 1 ug/ml.
Presentation
PBS, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee