Target
Rat SLC12A5 / KCC2
Synonyms
SLC12A5 | Erythroid K-Cl cotransporter 2 | HKCC2 | K-Cl cotransporter 2 | KCC2 | KIAA1176 | Neuronal K-Cl cotransporter
Reactivity
Rat, Dog
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
N-Terminus His-tag fusion protein corresponding to residues 932-1043 of rat K+/Cl- Cotransporter (KCC2).
Specificity
Recognizes rat K+/Cl- Cotransporter (KCC2), Mr ~140kD. An unknown band >200kD was also detected. Species cross-reactivity: Canine. Wide range of species cross-reactivity expected due to sequence homology.
Applications
- ICC (1:200)
- Western blot (1 µg/ml)
Usage
Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunocytochemistry: 1:200 detected KCC2 in MDCK cells as reported by an independent laboratory.
Presentation
0.07 M Tris-Glycine, pH 7.4, 0.105 M NaCl, 0.035% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee