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Catalog Number	Size	Price
LS-C139812-100	100 µg (0.5 mg/ml)	$503

SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

SIRT3 / Sirtuin 3 Antibody - SIRT3 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

SIRT3 / Sirtuin 3 Antibody - SIRT3 / Sirtuin 3 antibody flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

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Polyclonal Goat anti‑Mouse SIRT3 / Sirtuin 3 Antibody (aa170‑183, IF, WB) LS‑C139812

Name: Anti-SIRT3 / Sirtuin 3 Antibody | Goat anti-Mouse Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C139812-100
Price: 503.0000 USD
Availability: InStock

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Antibody:

SIRT3 / Sirtuin 3 Goat anti-Mouse Polyclonal (aa170-183) Antibody

Application:

IF, WB, Peptide-ELISA

Reactivity:

Mouse, Rat, Pig

Format:

Unconjugated, Unmodified

$503

LS-C139812-100

Toll Free North America

(800) 227-6666

For Research Use Only

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Overview

Antibody:

SIRT3 / Sirtuin 3 Goat anti-Mouse Polyclonal (aa170-183) Antibody

Application:

IF, WB, Peptide-ELISA

Reactivity:

Mouse, Rat, Pig

Format:

Unconjugated, Unmodified

Specifications

Description

Sirtuin 3 antibody LS-C139812 is an unconjugated goat polyclonal antibody to Sirtuin 3 (SIRT3) (aa170-183) from mouse. It is reactive with mouse, rat and pig. Validated for IF, Peptide-ELISA and WB.

Target

Mouse SIRT3 / Sirtuin 3

Synonyms

See All SIRT3 / Sirtuin 3 Antibodies

Host

Goat

Reactivity

Mouse, Rat, Pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Modifications

Unmodified

Immunogen

Peptide with sequence C-RASGIPASKLVEAH, from the internal region of the protein sequence according to NP_071878.2NP_001171275.1.

Epitope

aa170-183

Specificity

Mouse SIRT3. This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1

Applications

Immunofluorescence (10 µg/ml)
Western blot (1 - 3 µg/ml)
Peptide Enzyme-Linked Immunosorbent Assay (1:64000)

Usage

Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 30kD band observed in Mouse Heart lysates (calculated MW of 28.8kD according to NP_071878.2). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 15kD. This band was successfully blocked by incubation with the immunizing peptide.

Presentation

TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA

Storage

Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About SIRT3 / Sirtuin 3

Uniprot: Q9NTG7 NCBI: NM_012239 NP_036371.1