Target
Rat SFTPD / Surfactant Protein D
Synonyms
SFTPD | COLEC7 | Collectin 7 | Collectin-7 | Lung surfactant protein D | PSP-D | PSPD | SP-D | SFTP4 | Surfactant protein D
Reactivity
Rat, Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein A purified
Immunogen
Purified rat surfactant protein D from bronchoalveolar lavage.
Specificity
Recognizes an epitope localized in the lectin domain of rat surfactant protein D (SP-D). Reacts with alveolar type II cells, alveolar macrophages, types of Clara cells SP-A+ and SP-A- and extracellular deposits. Does not react with Alveolar type III cells. No reaction on rat skin, colon, kidney or liver. Species cross-reactivity: Rat and human.
Applications
- IHC
- IHC - Paraffin
- IHC - Frozen
- Western blot
- ELISA
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Usage
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Immunohistochemistry (Use snap frozen or paraffin embedded tissue): Fresh rat tissue: 1:400; fresh human tissue: 1:20. Fixation: Acetone 5 minutes at 4?C, paraformaldehyde, paraffin. Tissue pretreatment: Microwave treatment 2 x 5 minutes at 750W for paraffin tissues. ELISA: Use as a capture antibody.
Presentation
Lyophilized powder from PBS, pH 7.2, 5 mg/ml BSA, 0.09% sodium azide
Reconstitution
Reconstitute in deionized water
Storage
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFTPD / Surfactant Protein D