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Catalog Number Size Price
LS-C407829-10 10 µg $318 
LS-C407829-100 100 µg $470 
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5
SFTPD / Surfactant Protein D Antibody - Surfactant protein D antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPD / Surfactant Protein D Antibody - Surfactant protein D antibody Western blot. All lanes: Anti Surfactant protein D at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Predicted band size: 38 kD. Observed band size: 38 kD.
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in frozen section of human placenta tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. 5
SFTPD / Surfactant Protein D Antibody - Surfactant protein D antibody IHC-paraffin. IHC(P): Human Lung Cancer Tissue.
SFTPD / Surfactant Protein D Antibody - IHC analysis of Surfactant protein D using anti-Surfactant protein D antibody. Surfactant protein D was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Surfactant protein D Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SFTPD / Surfactant Protein D Antibody - Surfactant protein D antibody Western blot. All lanes: Anti Surfactant protein D at 0.5 ug/ml. Lane 1: Rat Lung Tissue Lysate at 50 ug. Lane 2: Rat Brain Tissue Lysate at 50 ug. Lane 3: PANC Whole Cell Lysate at 40 ug. Predicted band size: 38 kD. Observed band size: 38 kD.
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Polyclonal Rabbit anti‑Human SFTPD / Surfactant Protein D Antibody (aa292‑321, IHC, WB) LS‑C407829

Polyclonal Rabbit anti‑Human SFTPD / Surfactant Protein D Antibody (aa292‑321, IHC, WB) LS‑C407829

Antibody:
SFTPD / Surfactant Protein D Rabbit anti-Human Polyclonal (aa292-321) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407829-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SFTPD / Surfactant Protein D Rabbit anti-Human Polyclonal (aa292-321) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB, ELISA
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Surfactant Protein D antibody LS-C407829 is an unconjugated rabbit polyclonal antibody to Surfactant Protein D (SFTPD) (aa292-321) from human. It is reactive with human and rat. Validated for ELISA, ICC, IHC and WB.
Target
Human SFTPD / Surfactant Protein D
Synonyms
SFTPD | COLEC7 | Collectin 7 | Collectin-7 | Lung surfactant protein D | PSP-D | PSPD | SP-D | SFTP4 | Surfactant protein D
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human Surfactant protein D (292-321 aa RSAAENAALQQLVVAKNEAAFLSMTD SKTE), different from the related mouse and rat sequences by eight amino acids.
Epitope
aa292-321
Specificity
Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • IHC - Frozen
  • ICC
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SFTPD / Surfactant Protein D
P35247 NM_003019 NP_003010.4

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2024