LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > SF1 > LS-C801837
 
Print Page  
Catalog Number Size Price
LS-C801837-100 100 µl (1 mg/ml) $379 
LS-C801837-200 200 µl (1 mg/ml) $421 
SF1 Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SF1 Antibody - Western blot analysis Splicing factor 1 using COLO205 whole cells lysates
SF1 Antibody - Staining HuvEc cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
SF1 Antibody - Western blot analysis Splicing factor 1 using COLO205 whole cells lysates
1 of 2
2 of 2

Polyclonal Rabbit anti‑Human SF1 Antibody (IF, WB) LS‑C801837

Polyclonal Rabbit anti‑Human SF1 Antibody (IF, WB) LS‑C801837

Submit Review LSBio Guarantee
Antibody:
SF1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801837-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SF1 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
SF1 antibody LS-C801837 is an unconjugated rabbit polyclonal antibody to human SF1. Validated for IF, Peptide-ELISA and WB.
Target
Human SF1
Synonyms
SF1 | D11S636 | MBBP | ZCCHC25 | Transcription factor ZFM1 | Zinc finger gene in MEN1 locus | Zinc finger protein 162 | BBP | ZNF162 | Splicing factor 1 | ZFM1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human Splicing factor 1.
Specificity
Splicing factor 1 Antibody detects endogenous levels of total Splicing factor 1.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SF1
Q15637 NM_004630 NP_004621.2

Publications (0)

Submit a Publication for LS-C801837

Customer Reviews (0)

Submit a review for LS-C801837

Featured Products

SF1 Antibody - Anti-SF1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
PathPlus™ SF1 Antibody (clone 2E12) LS‑B4465
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
SF1 Antibody - SF1 antibody ARP41214_T100-NP_004621-SF1 (splicing factor 1) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SF1 Antibody (N‑Terminus) LS‑C31720
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
SF1 Antibody - Anti-SF1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ SF1 Antibody (aa48‑97) LS‑B7622
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
SF1 Antibody - IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-splicing factor 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SF1 Antibody (aa11‑30) LS‑C313004
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
SF1 Antibody - Immunohistochemical analysis of SF1 (pS82) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
SF1 Antibody (N‑Terminus) LS‑C358857
Species: Human, Mouse, Rat, Bovine, Rabbit, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
SF1 Protein - 12.5% SDS-PAGE of human SF1 stained with Coomassie Blue
Human SF1 Protein (Recombinant GST) (Full Length) - LS-G30928
Source: Wheat
Tag: GST
See all SF1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/20/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com