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Vector Laboratories, Inc.
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Catalog Number Size Price
LS-C313004-10 10 µg $318 
LS-C313004-100 100 µg $470 
SF1 Antibody - IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-splicing factor 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SF1 Antibody - SF1 antibody. IHC(F): Rat Spleen Tissue.
SF1 Antibody - SF1 antibody. IHC(P): Human Lung Cancer Tissue.
SF1 Antibody - SF1 antibody. ICC: HELA Cell.
SF1 Antibody - Anti-splicing factor 1 antibody, Western blotting All lanes: Anti splicing factor 1 at 0.5ug/ml Lane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KD Observed bind size: 70KD
SF1 Antibody - WB of SF1 antibody. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: PANC Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SW620 Cell Lysate. Lane 6: SKOV Cell Lysate.
SF1 Antibody - IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody. splicing factor 1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-splicing factor 1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
SF1 Antibody - SF1 antibody. IHC(F): Rat Spleen Tissue.
SF1 Antibody - SF1 antibody. IHC(P): Human Lung Cancer Tissue.
SF1 Antibody - SF1 antibody. ICC: HELA Cell.
SF1 Antibody - Anti-splicing factor 1 antibody, Western blotting All lanes: Anti splicing factor 1 at 0.5ug/ml Lane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugLane 3: PANC Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugLane 5: SW620 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 52KD Observed bind size: 70KD
SF1 Antibody - WB of SF1 antibody. Lane 1: Rat Spleen Tissue Lysate. Lane 2: Rat Liver Tissue Lysate. Lane 3: PANC Cell Lysate. Lane 4: COLO320 Cell Lysate. Lane 5: SW620 Cell Lysate. Lane 6: SKOV Cell Lysate.
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Polyclonal Rabbit anti‑Human SF1 Antibody (aa11‑30, IHC, WB) LS‑C313004

Polyclonal Rabbit anti‑Human SF1 Antibody (aa11‑30, IHC, WB) LS‑C313004

Note: This antibody replaces LS-C389230
Antibody:
SF1 Rabbit anti-Human Polyclonal (aa11-30) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313004-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SF1 Rabbit anti-Human Polyclonal (aa11-30) Antibody
Application:
IHC, IHC-P, IHC-Fr, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
SF1 antibody LS-C313004 is an unconjugated rabbit polyclonal antibody to SF1 (aa11-30) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human SF1
Synonyms
SF1 | D11S636 | MBBP | ZCCHC25 | Transcription factor ZFM1 | Zinc finger gene in MEN1 locus | Zinc finger protein 162 | BBP | ZNF162 | Splicing factor 1 | ZFM1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the N-Terminus of human splicing factor 1(11-30 aa DFPSKKRKRSRWNQDTMEQK), identical to the related rat and mouse sequences.
Epitope
aa11-30
Specificity
Detected in lung, ovary, adrenal gland, colon, kidney, muscle, pancreas, thyroid, placenta, brain, liver and heart.
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SF1
Q15637 NM_004630 NP_004621.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/19/2024