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Antibodies > SERPINF1 / PEDF > LS-C809550
 
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Catalog Number Size Price
LS-C809550-100 100 µl (1 mg/ml) $379 
LS-C809550-200 200 µl (1 mg/ml) $421 
SERPINF1 / PEDF Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SERPINF1 / PEDF Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
SERPINF1 / PEDF Antibody - Western blot analysis of extracts of various samples using SERPINF1 antibody. Lane 1: mouse brain; Lane 2: HeLa;
SERPINF1 / PEDF Antibody - Western blot analysis of extracts of human liver tissue using SERPINF1 antibody. The lane on the left is treated with the antigen-specific peptide.
SERPINF1 / PEDF Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
SERPINF1 / PEDF Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
SERPINF1 / PEDF Antibody - Western blot analysis of extracts of various samples using SERPINF1 antibody. Lane 1: mouse brain; Lane 2: HeLa;
SERPINF1 / PEDF Antibody - Western blot analysis of extracts of human liver tissue using SERPINF1 antibody. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human SERPINF1 / PEDF Antibody (IHC, IF, WB) LS‑C809550

Polyclonal Rabbit anti‑Human SERPINF1 / PEDF Antibody (IHC, IF, WB) LS‑C809550

Submit Review LSBio Guarantee
Antibody:
SERPINF1 / PEDF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C809550-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
SERPINF1 / PEDF Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PEDF antibody LS-C809550 is an unconjugated rabbit polyclonal antibody to PEDF (SERPINF1) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human SERPINF1 / PEDF
Synonyms
SERPINF1 | EPC-1 | OI12 | PEDF | Serpin F1 | PIG35 | OI6
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human SERPINF1.
Specificity
SERPINF1 Antibody detects endogenous levels of total SERPINF1.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINF1 / PEDF
P36955 NM_002615 NP_002606.3

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PathPlus™ SERPINF1 / PEDF Antibody (aa196‑347) LS‑B2471
Species: Human
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, ELISA
SERPINF1 / PEDF Antibody - Anti-PEDF antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 8 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
SERPINF1 / PEDF Antibody (N‑Terminus) LS‑B2505
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
SERPINF1 / PEDF Antibody - Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with SERPINF1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
SERPINF1 / PEDF Antibody (aa9‑36) LS‑C100901
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Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
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Species: Human
Applications: Western blot, ELISA
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Species: Human
Applications: Western blot, ELISA
See all SERPINF1 / PEDF Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For questions about orders:
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