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Catalog Number Size Price
LS-C357544-10 10 µg $318 
LS-C357544-100 100 µg (0.5 mg/ml) $470 
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Human Mammary Cancer Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Rat Intestine Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Mouse Intestine Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody ICC: MCF-7 Cell.
SERPINB5 / Maspin Antibody - Western blot analysis of MASPIN using anti-MASPIN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates, Lane 3: human placenta tissue lysates, Lane 4: human MDA-MB-231 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42KD. The expected band size for MASPIN is at 42KD.
SERPINB5 / Maspin Antibody - MASPIN antibody Western blot. All lanes: Anti MASPIN at 0.5 ug/ml. Lane 1: Mouse Intestine Tissue Lysate at 50 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Human Mammary Cancer Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Rat Intestine Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody IHC-paraffin: Mouse Intestine Tissue.
SERPINB5 / Maspin Antibody - MASPIN antibody ICC: MCF-7 Cell.
SERPINB5 / Maspin Antibody - Western blot analysis of MASPIN using anti-MASPIN antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat small intestine tissue lysates, Lane 2: mouse small intestine tissue lysates, Lane 3: human placenta tissue lysates, Lane 4: human MDA-MB-231 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MASPIN antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MASPIN at approximately 42KD. The expected band size for MASPIN is at 42KD.
SERPINB5 / Maspin Antibody - MASPIN antibody Western blot. All lanes: Anti MASPIN at 0.5 ug/ml. Lane 1: Mouse Intestine Tissue Lysate at 50 ug. Lane 2: Human Placenta Tissue Lysate at 50 ug. Lane 3: MCF-7 Whole Cell Lysate at 40 ug. Lane 4: MM231 Whole Cell Lysate at 40 ug. Predicted band size: 42 kD. Observed band size: 42 kD.
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Polyclonal Rabbit anti‑Human SERPINB5 / Maspin Antibody (aa1‑350, IHC, WB) LS‑C357544

Polyclonal Rabbit anti‑Human SERPINB5 / Maspin Antibody (aa1‑350, IHC, WB) LS‑C357544

Antibody:
SERPINB5 / Maspin Rabbit anti-Human Polyclonal (aa1-350) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C357544-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
SERPINB5 / Maspin Rabbit anti-Human Polyclonal (aa1-350) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Maspin antibody LS-C357544 is an unconjugated rabbit polyclonal antibody to Maspin (SERPINB5) (aa1-350) from human. It is reactive with human, mouse and rat. Validated for ICC, IHC and WB.
Target
Human SERPINB5 / Maspin
Synonyms
SERPINB5 | Maspin | Peptidase inhibitor 5 | PI5 | Protease inhibitor 5 (maspin) | Serpin B5 | PI-5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.
Epitope
aa1-350
Specificity
Normal mammary epithelial cells.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About SERPINB5 / Maspin
P36952 NM_002639 NP_002630.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/13/2024