Human SEMA4D / Semaphorin 4D / CD100

Mouse

Human (tested or 100% immunogen sequence identity)

Horse

IgM,k Monoclonal

133-1C6

Unconjugated

PEG purified

Unmodified. Also available Azide-free, BSA-free.

PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

Human SEMA4D / Semaphorin 4D / CD100

• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI. Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation. Optimal dilution of the Semaphorin-4D antibody to be determined by the researcher.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q92854 NCBI: NM_006378 NP_006369.3