Human S100 Protein

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

6G1

Unconjugated

Affinity purified

Unmodified

Human brain S100 protein.

Recognizes S100 protein derived from human brain tissue. It is a 21kD acidic calcium-binding protein synthesized by astroglial cells. It can be found predominantly as two isoforms, the alpha-beta heterodimer (S100a) and the beta-beta homodimer (S100b). The S100 family of proteins have been implicated in a large variety of intracellular and extracellular regulatory activities, such as cellular energy metabolism, cytoskeleton modification, cell proliferation and differentiation. When glial cells are damaged, S100 protein is leaked into the extracellular matrix and cerebrospinal fluid, further releasing into the bloodstream. As such, S100 protein can be used as a sensitive and reliable marker for nervous system damage, such as after brain damage or acute stroke. Recognizes both S100a (alpha-beta) and S100b (beta-beta). May be used to detect S100 protein in ELISA assay. Sensitive to EDTA and other bivalent-binding agents. Better performance can be obtained in the presence of 5 mM CaCl2 in assay buffers.

• Western blot
• ELISA

ELISA: This product may be used in an indirect ELISA or as a detection antibody in a sandwich ELISA together with LS-C58309 as a capture reagent.

PBS, 0.09% Sodium Azide

Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

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