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Catalog Number Size Price
LS-B47-50 50 µg (1 mg/ml) $535 
S100 Protein Antibody - Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
S100 Protein Antibody - Anti-S-100 Antibody - Western Blot. Western blot of Affinity Purified anti-S-100 antibody shows detection of a band ~11 kD corresponding to bovine S-100 monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
S100 Protein Antibody - Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
S100 Protein Antibody - Anti-S-100 Antibody - Western Blot. Western blot of Affinity Purified anti-S-100 antibody shows detection of a band ~11 kD corresponding to bovine S-100 monomer (100 ng loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). Approximately 35 ug of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1000 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
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IHC‑plus™ Polyclonal Rabbit anti‑Bovine S100 Protein Antibody (IHC, WB) LS‑B47

IHC‑plus™ Polyclonal Rabbit anti‑Bovine S100 Protein Antibody (IHC, WB) LS‑B47

Note: This antibody replaces LS-C18888
Antibody:
S100 Protein Rabbit anti-Bovine Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$535
LS-B47-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
S100 Protein Rabbit anti-Bovine Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
S100 Protein antibody LS-B47 is an unconjugated rabbit polyclonal antibody to bovine S100 Protein. Validated for ELISA, IHC and WB.
Target
Bovine S100 Protein
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Dog, Primate (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
Specificity
A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B47 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B47 was determined to be 2.5 ug/ml.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

S100 Protein Antibody - Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-S100 Protein antibody IHC of human prostate, nerve. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ S100 Protein Antibody for IHC, WB/Western, ELISA LS-B47 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024