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| Catalog Number | Size | Price |
|---|---|---|
| LS-C757218-300 | 300 µg (3 mg/ml) | $422 |
Polyclonal Goat anti‑All species RFP / Red Fluorescent Protein Antibody (IHC, IF, WB) LS‑C757218
Polyclonal Goat anti‑All species RFP / Red Fluorescent Protein Antibody (IHC, IF, WB) LS‑C757218
Antibody:
RFP / Red Fluorescent Protein Goat Polyclonal Antibody
Application:
IHC-P, IHC-Fr, IF, WB
Reactivity:
All species
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
RFP / Red Fluorescent Protein Goat Polyclonal Antibody
Application:
IHC-P, IHC-Fr, IF, WB
Reactivity:
All species
Format:
Unconjugated, Unmodified
Specifications
Description
Red Fluorescent Protein antibody LS-C757218 is an unconjugated goat polyclonal antibody to all species Red Fluorescent Protein (RFP). Validated for IF, IHC and WB.
Host
Goat
Reactivity
All species
Predicted
All species (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
epitope affinity purified
Modifications
Unmodified
Immunogen
Purified recombinant RFP peptide produced in E. coli.
Specificity
In 293HEK cells transfected with cds plasmid detects a band of 29 kDa by Western blot. This antibody also recognizes very well tdTomato and does not cross-react to GFP (green fluorescent protein).
Applications
- IHC - Paraffin (1:50 - 1:500)
- IHC - Frozen (1:50 - 1:500)
- Immunofluorescence (1:50 - 1:500)
- Western blot (1:500 - 1:2000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The antibody solution should be gently mixed before use.
Presentation
Supplied as a 100 µl (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
Storage
Short-term store at 2°C to 8°C. Long term store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Discosoma
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Discosoma
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot
![RFP / Red Fluorescent Protein Antibody - Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/rfp-red-fluorescent-protein-antibody-fitc-ls-c154267/590804_5351757.png)
Species:
Discosoma
Applications:
Immunofluorescence, Western blot, Fluorophore-Linked Immunosorbent Assay
Species:
Discosoma
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/23/2025
Date Requested: 1/23/2025
