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| Catalog Number | Size | Price |
|---|---|---|
| LS-C17622-100 | 100 µg | $854 |
Polyclonal Chicken anti‑Discosoma RFP / Red Fluorescent Protein Antibody (WB) LS‑C17622
Polyclonal Chicken anti‑Discosoma RFP / Red Fluorescent Protein Antibody (WB) LS‑C17622
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
Red Fluorescent Protein antibody LS-C17622 is an unconjugated chicken polyclonal antibody to discosoma Red Fluorescent Protein (RFP). Validated for WB.
Host
Chicken
Reactivity
Discosoma
(tested or 100% immunogen sequence identity)
Clonality
IgY
Polyclonal
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant red fluorescent protein (RFP) highly purified by affinity chromatography on Nickel column.
Specificity
Recognizes Red fluorescent protein (RFP). Shows no reactivity to GFP.
Applications
- Western blot (1:10000 - 1:30000)
Usage
Suitable for use in Western Blot. Western Blot: 1:10000-1:30000 when detected with an anti-chicken horseradish peroxidase-conjugated secondary by ECL or by a colorimetric assay using DAB.
Presentation
10 mM Tris, pH 7.5, 100 mM sodium chloride, 0.02% sodium azide.
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Discosoma
Applications:
IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Species:
Discosoma
Applications:
Western blot, Immunoprecipitation, ELISA
Species:
Applications:
ICC, Western blot
![RFP / Red Fluorescent Protein Antibody - Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody. Lane 1 shows no reaction against a GFP recombinant protein present in 10 ug of HeLa cell extract. Lane 2 shows a single band detected in 10 ug of a HeLa lysate containing RFP recombinant protein. polyclonal anti-RFP detects a 27 kDa band corresponding to the epitope tag RFP. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted 1:2,500 for 1 h at room temperature followed by washes and reaction with a 1:5,000 dilution of IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] MX. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com//image2/rfp-red-fluorescent-protein-antibody-fitc-ls-c154267/590804_5351757.png)
Species:
Discosoma
Applications:
Immunofluorescence, Western blot, Fluorophore-Linked Immunosorbent Assay
Species:
Discosoma
Applications:
Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/23/2025
Date Requested: 1/23/2025
