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Catalog Number Size Price
LS-C820476-100 100 µl $302 
Requiem / DPF2 Antibody - Immunofluorescence staining of DPF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DPF2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.
Requiem / DPF2 Antibody - Anti-DPF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC-12 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: NIH-3T3 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.
Requiem / DPF2 Antibody - DPF2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-DPF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DPF2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.
Requiem / DPF2 Antibody - Immunofluorescence staining of DPF2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human DPF2 polyclonal antibody (dilution ratio 1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.
Requiem / DPF2 Antibody - Anti-DPF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: PC-12 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: NIH-3T3 Whole Cell Lysate. Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.
Requiem / DPF2 Antibody - DPF2 was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 4 uL anti-DPF2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-DPF2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa. Observed band size: 44 kDa.
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Polyclonal Rabbit anti‑Human Requiem / DPF2 Antibody (IF, WB) LS‑C820476

Polyclonal Rabbit anti‑Human Requiem / DPF2 Antibody (IF, WB) LS‑C820476

Antibody:
Requiem / DPF2 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$302
LS-C820476-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Requiem / DPF2 Rabbit anti-Human Polyclonal Antibody
Application:
ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DPF2 antibody LS-C820476 is an unconjugated rabbit polyclonal antibody to human DPF2 (Requiem). Validated for ICC, IF, IP and WB.
Target
Human Requiem / DPF2
Synonyms
DPF2 | BAF45D | REQ | UBID4 | Zinc finger protein ubi-d4 | BRG1-associated factor 45D | Ubi-d4 | Protein requiem | Requiem
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
E. coli-derived Human DPF2 fragment
Specificity
Human DPF2
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.0, 0.03% Proclin 300
Storage
Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Requiem / DPF2
Q92785 NM_006268 NP_006259.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/17/2025