LSBio logo with text
Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers


Customer Service: (800) 227-6666 / (650) 697-3600


Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > RELA / NFKB p65 > LS-C800713
 
Print Page  
Catalog Number Size Price
LS-C800713-100 100 µl (1 mg/ml) $379 
LS-C800713-200 200 µl (1 mg/ml) $421 
RELA / NFKB p65 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RELA / NFKB p65 Antibody - Western blot analysis of NF-kappaB p65 phosphorylation expression in IL-1 treated RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RELA / NFKB p65 Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human liver tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat ovarian tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - 1:100 staining human heart tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RELA / NFKB p65 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RELA / NFKB p65 Antibody - Western blot analysis of NF-kappaB p65 phosphorylation expression in IL-1 treated RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
1 of 11
2 of 11
3 of 11
4 of 11
5 of 11
6 of 11
7 of 11
8 of 11
9 of 11
10 of 11
11 of 11

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser536, IHC, IF, WB) LS‑C800713

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser536, IHC, IF, WB) LS‑C800713

Submit Review LSBio Guarantee
Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800713-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer536) Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-C800713 is an unconjugated rabbit polyclonal antibody to NFKB p65 (RELA) (pSer536) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human NF- kappaB p65 around the phosphorylation site of Serine 536.
Epitope
pSer536
Specificity
Phospho-NF- kappaB p65 (Ser536) Antibody detects endogenous levels of NF- kappaB p65 only when phosphorylated at Serine 536.
Applications
  • IHC (1:50 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Immunoprecipitation (1:100 - 1:500)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, Sodium Azide, Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

Publications (0)

Submit a Publication for LS-C800713

Customer Reviews (0)

Submit a review for LS-C800713

Featured Products

RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ RELA / NFKB p65 Antibody (phospho‑Ser529) LS‑B652
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ RELA / NFKB p65 Antibody (phospho‑Ser536) LS‑B53
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
RELA / NFKB p65 Antibody (aa297‑308) LS‑C9296
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse
Applications: IHC, Immunofluorescence, Western blot, ELISA
RELA / NFKB p65 Antibody - Western blot of Acetyl-NFkappaB-p65 (K122) antibody
RELA / NFKB p65 Antibody (Acetyl‑Lys122) LS‑C387648
Species: Human, Mouse, Rat
Applications: Western blot, ELISA
RELA / NFKB p65 Antibody - Anti-RELA antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
IHC‑plus™ RELA / NFKB p65 Antibody (C‑Terminus) LS‑B653
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA, Chromatin Immunoprecipitation, Gel shift
RELA / NFKB p65 Antibody - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue.
RELA / NFKB p65 Antibody (phospho‑Ser276) LS‑C335799
Species: Human
Applications: IHC, Immunofluorescence, Western blot
See all RELA / NFKB p65 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2024 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

Orders:
customerservice@vectorlabs.com

For technical assistance:
Technical.Support@LSBio.com