Human RELA / NFKB p65

Rabbit

Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

A synthetic peptide, RIEEKRKRTYET, corresponding to the NLS of the human NFkB p65 protein conjugated to KLH using maleimide. A residue of cysteine was added to the N-Terminus end to facilitate coupling. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%); Paramecium (83%).

aa297-308

Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.

• IHC (1:100)
• Immunofluorescence (1:200)
• Western blot (1:1000)
• ELISA (1:100 - 1:1000)

Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Western Blot: 1:1000. Immunohistochemistry: 1:100. Immunofluorescence microscopy: 1:100. Super shift: 0.5-1 ul/assay.

PBS, pH 7.2, 0.1% Sodium Azide, 40% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3