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Catalog Number Size Price
LS-C154220-100 100 µg (1 mg/ml) $567 
RELA / NFKB p65 Antibody - anti-NFkB antibody. Affinity purified Anti-NFKB p65 (Rel A) pS276 Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 600-401-264 lot 24040 was probed against Normal (Lane 1) and TNFalpha (Lane 2) Stimulated HeLa whole cell lysates. A band was observed between 55 and 72 kD corresponding to the expected MW of NFkB p65. The observed higher MW bands have not been characterized.

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser276, IHC, WB) LS‑C154220

Polyclonal Rabbit anti‑Human RELA / NFKB p65 Antibody (phospho‑Ser276, IHC, WB) LS‑C154220

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer276) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C154220-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
RELA / NFKB p65 Rabbit anti-Human Polyclonal (pSer276) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
NFKB p65 antibody LS-C154220 is an unconjugated rabbit polyclonal antibody to human NFKB p65 (RELA) (pSer276). Validated for ELISA, IHC, IP and WB. Cited in 1 publication.
Target
Human RELA / NFKB p65
Synonyms
RELA | NF-kappa-B p65delta3 | NF-kappaB | NFKB3 | p65 | Transcription factor p65 | NF kappaB | NFKB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
pSer276
Specificity
This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.
Applications
  • IHC
  • Western blot (1:1000)
  • Immunoprecipitation
  • ELISA (1:5000 - 1:25000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1000 dilution is recommended. A 65 kD band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RELA / NFKB p65
Q04206 NM_021975 NP_068810.3

LSBio Ratings

RELA / NFKB p65 Antibody (phospho-Ser276) for IHC, WB/Western, IP, ELISA LS-C154220 has an LSBio Rating of
Publications (4)

Publications (1)

Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation. Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB. Journal of immunology (Baltimore, Md. : 1950). 2002 169:6490-7.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/3/2024