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Catalog Number	Size	Price
LS-C413576-100	100 µl (1 mg/ml)	$321

RELA / NFKB p65 Antibody - Immunohistochemical analysis of NF-kappaB p65 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

RELA / NFKB p65 Antibody - Immunofluorescent analysis of NF-kappaB p65 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

RELA / NFKB p65 Antibody - Western blot analysis of NF-kappaB p65 expression in HeLa (A); mouse spleen (B); rat heart (C) whole cell lysates.

RELA / NFKB p65 Antibody - Immunoprecipitation of NF-kappaB p65 from 0.5mg HeLa whole cell extract lysate; using Anti-NF-kappaB p65 Antibody.

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Monoclonal Mouse anti‑Human RELA / NFKB p65 Antibody (IHC, IF, WB) LS‑C413576

Name: Anti-RELA / NFKB p65 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C413576-100
Price: 321.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$321

LS-C413576-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

NFKB p65 antibody LS-C413576 is an unconjugated mouse monoclonal antibody to NFKB p65 (RELA) from human. It is reactive with human, mouse and rat. Validated for ICC, IF, IHC, IP and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human RELA / NFKB p65

Synonyms

See All RELA / NFKB p65 Antibodies

Host

Mouse

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

Monoclonal

Conjugations

Unconjugated

Purification

Purified

Modifications

Unmodified

Immunogen

Recombinant protein corresponding to human NF-kappaB p65.

Specificity

Recognizes endogenous levels of NF-kappaB p65 protein.

Applications

IHC
IHC - Paraffin (1:200 - 1:500)
ICC (1:100 - 1:200)
Immunofluorescence (1:100 - 1:200)
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:100 - 1:200)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RELA / NFKB p65

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3

LSBio Ratings

RELA / NFKB p65 Antibody for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-C413576 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Adiponectin inhibits the activation of lung fibroblasts and pulmonary fibrosis by regulating the nuclear factor kappa B (NF-?B) pathway. Xin Wang, Jian Yang, Liangquan Wu, Chunran Tong, Ying Zhu, Wei Cai, Bing Wan, Xiuwei Zhang. Bioengineered. 2022 April;13:10098-10110.

Full Text Article PubMed: 35435119 PMC: PMC9162013

Submit a Publication for LS-C413576