Human RELA / NFKB p65

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Affinity purified

Unmodified

This antibody was purified from whole rabbit serum prepared by repeated immunizations with the NFkB p65 peptide corresponding to the NLS of the human protein conjugated to KLH using maleimide.  A residue of cysteine was added to the amino terminal end to facilitate coupling.

The antibody selectively binds to the activated form of NFkB. Anti-NFkB p65 NLS may react non-specifically with other proteins.

• IHC
• IHC - Paraffin (1:200)
• Immunofluorescence (1:200)
• Western blot (1:2000)
• ELISA (1:5000 - 1:25000)
• Gel shift

Suitable for immunohistochemistry, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against NFxB p65 NLS at a dilution of 1:2000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NFxB NLS is suitable for the gel supershift assay and was found to be reactive against all p65 (Rel A) containing human NFxB complexes using 0.5 to 1.0 ul per assay.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3