Products

Research Areas

COVID-19

Resources

Distributors

Quick Order

Cart

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Cart

Products

Antibodies

All Antibodies Primary Antibodies Secondary Antibodies IHC-plus™ Antibodies PathPlus™ Antibodies Isotype Control Antibodies

ELISA and Assay Kits

All Kits Assay Kits Traditional ELISA Kits Cell-Based ELISA Kits DNA-Binding ELISA Kits Phospho-Specific ELISA Kits ELISA Development Kits Chemiluminescent CLIA Kits

Proteins

All Proteins Recombinant Proteins Native Proteins Over-Expression Lysates Bio-Active Proteins Animal-Free Proteins Synthetic Peptides Cell and Tissue Lysates

Research Areas

Cancer Research

Breast Cancer IHC Colorectal Cancer IHC Lung Cancer IHC Prostate Cancer IHC

Pathways And Groups

Apoptosis Caspases Chemokines

Infectious Disease

Virology Coronaviridae (COVID-19) Hepatitis Viruses Herpesviridae (Herpes) Orthomyxoviridae (Influenza) Papillomaviridae (HPV) Paramyxoviridae (Mumps) Pneumoviridae (RSV) Polyomaviridae (SV40) Retroviridae (HIV) Togaviridae (Measles)

Neurodegenerative Disease

Alzheimer's Disease

COVID-19

Coronavirus Detection Coronavirus and COVID-19 Inhibitors Coronavirus Antibodies, Proteins, and cDNAs Cytokine Release Syndrome General Coronavirus Information

Resources

Purchasing

Product Ordering Terms and Conditions Holiday Schedule About the LSBio Guarantee About Reviews

Reference Material

About LSBio (LifeSpan BioSciences Inc.) About Our IHC Validation Process About Our IHC-plus™ Immunohistochemistry Protocol About Our Protocols LSBio ELISA Kit Development Guide Media

Secure Logins

How To Buy

Location

Corporate Headquarters

Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Telephone Numbers

Customer Service: (800) 227-6666 / (650) 697-3600

Contact Us

Additional Contact Details

Distributors

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Forgot password?

Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.

Login Name*:
Email*:
Password*:
Confirm Password*:
Name:
Organization:
Role:

Fields marked with a * are required.

Quick Order

Catalog Number	Size	Price
LS-C153771-100	100 µg (0.98 mg/ml)	$574

RELA / NFKB p65 Antibody - Monoclonal anti NFKB p65 (Rel A) antibody was used to detect p65 by immunofluorescence at a dilution of 1:5000. HeLa cells were grown to sub-confluent on 18 mm2 glass coverslips #1.5. Cells were either unstimulated (A), or stimulated (B) with 50 ng/ml of TNF alpha for 30 min prior fixation. Cells were then fixed in methanol and blocked with 10% normal goat serum (NGS), in PBS, and Triton X 0.2% (Tx) and incubated for 1 hr at RT with primary ab, counterstained with DAPI and washed in PBS/NGS/Tx. Cells were incubated for 1 hr at RT with Atto 425 conjugated anti mouse secondary antibody for STED CW imaging. Data was collected on a STED-CW TCS-SP5 Confocal system equipped with a DFC 350FX camera allowing sequential acquisition in wide field, confocal and STED CW imaging on the same system.

RELA / NFKB p65 Antibody - anti NFKB p65 (Rel A) monoclonal antibody Anti-NFKB p65 (Rel A) (MOUSE) Monoclonal Antibody - 200-301-065 was used to detect ~65 kD band (red arrow) in HeLa whole cell lysate. Lysate was run on 4-20% gradient gel transferred under standard conditions and blocked in 1% BSA-TTBS 30 min RT. Blot was probed with monoclonal anti p65 1:1000 in 1% BSA-TBS-T o/n 4? and detected with HRP conjugated Rb-anti Mouse antibody 1:40000 in MB-070 30 min RT.

1 of 2

2 of 2

Monoclonal Mouse anti‑Human RELA / NFKB p65 Antibody (clone 27F9.G4, C‑Terminus, IHC, IF, WB) LS‑C153771

Name: Anti-RELA / NFKB p65 Antibody | Mouse anti-Human Monoclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C153771-100
Price: 574.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

(4.0) Rating

Submit Review (1) Publication(s) LSBio Guarantee

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal (C-Terminus) (27F9.G4) Antibody

Application:

IHC, ICC, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

$574

LS-C153771-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

RELA / NFKB p65 Mouse anti-Human Monoclonal (C-Terminus) (27F9.G4) Antibody

Application:

IHC, ICC, IF, WB, ELISA

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

NFKB p65 antibody LS-C153771 is an unconjugated mouse monoclonal antibody to human NFKB p65 (RELA) (C-Terminus). Validated for ELISA, ICC, IF, IHC and WB. Cited in 1 publication.

(1) Publication(s)

Target

Human RELA / NFKB p65

Synonyms

See All RELA / NFKB p65 Antibodies

Host

Mouse

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

IgG2a,k Monoclonal

Clone

27F9.G4

Conjugations

Unconjugated

Purification

Protein A affinity chromatography

Modifications

Unmodified

Immunogen

NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Epitope

C-Terminus

Applications

IHC (1:200 - 1:600)
ICC
Immunofluorescence (1:5000)
Western blot (1:1000 - 1:5000)
ELISA (1:50000 - 1:100000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

Anti-NF-kB p65 Antibody is a mouse monoclonal antibody directed against NFkB p65 (Rel A) and recognizes a 65 kD band by Western blot against HeLa whole cell lysate. Control peptide (100-4165p) is sold separately.

Presentation

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About RELA / NFKB p65

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3

LSBio Ratings

RELA / NFKB p65 Antibody (C-Terminus, clone 27F9.G4) for IHC, ICC, IF/Immunofluorescence, WB/Western, ELISA LS-C153771 has an LSBio Rating of

(4.0) Rating

Publications (4)

Learn more about The LSBio Ratings Algorithm

Publications (1)

Her2 activates NF-kappaB and induces invasion through the canonical pathway involving IKKalpha. Merkhofer EC, Cogswell P, Baldwin AS. Oncogene. 2010 29:1238-48.

PubMed: 19946332 PMC: PMC2829103

Submit a Publication for LS-C153771