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Catalog Number	Size	Price
LS-C352774-100	100 µl (1 mg/ml)	$299

RB1 / Retinoblastoma / RB Antibody - Immunohistochemical analysis of RB1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

RB1 / Retinoblastoma / RB Antibody - Immunofluorescent analysis of RB1 staining in COLO205 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

RB1 / Retinoblastoma / RB Antibody - Western blot analysis of RB1 expression in Jurkat (A); COLO205 (B); HUVEC (C); A431 (D); NIH3T3 (E) whole cell lysates.

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Polyclonal Rabbit anti‑Human RB1 / Retinoblastoma / RB Antibody (C‑Terminus, IHC, IF, WB) LS‑C352774

Name: Anti-RB1 / Retinoblastoma / RB Antibody | Rabbit anti-Human | LSBio
Brand: Lifespan BioSciences
SKU: LS-C352774-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

RB1 / Retinoblastoma / RB Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Pig

Format:

Unconjugated, Unmodified

$299

LS-C352774-100

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Overview

Antibody:

RB1 / Retinoblastoma / RB Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Mouse, Rat, Bovine, Dog, Pig

Format:

Unconjugated, Unmodified

Specifications

Description

RB antibody LS-C352774 is an unconjugated rabbit polyclonal antibody to RB (RB1 / Retinoblastoma) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.

Target

Human RB1 / Retinoblastoma / RB

Synonyms

RB1 | p105-Rb | Osteosarcoma | PRb | Retinoblastoma | Retinoblastoma 1 | OSRC | RB | Pp110

See All RB1 / Retinoblastoma / RB Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat, Bovine, Dog, Pig (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human RB1.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of RB1 protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About RB1 / Retinoblastoma / RB

Uniprot: P06400 NCBI: NM_000321 NP_000312.2