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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60797-1 | 1 mg (1 mg/ml) | $417 |
Polyclonal Rabbit Anti‑Mouse IgG2a Secondary Antibody (AP, IHC, WB) LS‑C60797
Polyclonal Rabbit Anti‑Mouse IgG2a Secondary Antibody (AP, IHC, WB) LS‑C60797
Antibody:
Mouse IgG2a Rabbit anti-Mouse Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Alkaline Phosphatase, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
Mouse IgG2a Rabbit anti-Mouse Polyclonal (AP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Mouse
Format:
Alkaline Phosphatase, Unmodified
Specifications
Description
Mouse IgG2a antibody LS-C60797 is an AP-conjugated rabbit polyclonal antibody. Validated for ELISA, IHC and WB.
Host
Rabbit
Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Alkaline Phosphatase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Mouse IgG2a heavy chain.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Applications
- IHC (1:200 - 1:1000)
- Western blot (1:500 - 1:2500)
- ELISA (1:2000 - 1:10000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.1% Sodium Azide, 50% (v/v) Glycerol
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
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Featured Products
Species:
Mouse
Applications:
IHC - Paraffin, IHC - Frozen, Western blot, ELISA
Species:
Mouse
Applications:
ELISA
Species:
Mouse
Applications:
IHC, Western blot, ELISA
Species:
Mouse
Applications:
Immunofluorescence, Western blot, Flow Cytometry, Fluorophore-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/25/2024
Date Requested: 11/25/2024
