Human IgG Fab'2

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Alkaline Phosphatase

Affinity chromatography

Unmodified

Human IgG F(ab')2 fragment

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.

• IHC (1:200 - 1:1000)
• Western blot (1:500 - 1:2500)
• ELISA (1:2000 - 1:10000)

Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-anti body based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Human IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:2500 to 1:11000 of the stated concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol

Store at 4°C. Do not freeze.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.