Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG F(ab')2
Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Immunogen
Anti-Human IgG F(ab')2 fragment antibody was produced by repeated immunization with Human IgG F(ab')2 fragment in goat.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(c) or Bovine, Horse, Mouse and Rat Serum Proteins.
Applications
- IHC (1:500 - 1:2500)
- Western blot (1:1000 - 1:5000)
- ELISA (1:10000 - 1:40000)
- Dot Blot
- Electron Microscopy
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Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee