Human PVR / CD155

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC6A3

Unconjugated. Also available conjugated with Biotin, FITC, PE.

Protein A purified

Unmodified. Also available Preservative Free.

Recombinant Human CD155.

Antibody ANC6A3 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

• Flow Cytometry
• ELISA

Five x 105 cultured human U-937 cells per tube were washed and pre incubated ~5 minutes with 20 ul human IgG at 300 ug/ml (to reduce non specific binding ) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD155/Biotin at a concentration of 10 ug/ml. Cells were washed twice and incubated with 50 ul of 2o reagent GAM/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.09 log10 fluorescent units when compared to a Mouse IgG1/Biotin Isotype control. This binding was blocked when reagent was pre incubated with 10 0 ug/ml recombinant CD155-muIg.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.5 mg/ml Gentamicin

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15151 NCBI: NM_006505 NP_006496.4