Human PVR / CD155

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1,k Monoclonal

ANC6A3

Biotin. Also available Unconjugated or conjugated with FITC, PE.

Protein A purified

Unmodified

Recombinant Human CD155.

Antibody ANC6A3 binds to cell surface CD155 on U-937 cells, and binds to recombinant CD155 in EIA.

• Flow Cytometry
• ELISA
• Applications tested for the base form of this product only

Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated overnight in culture with 5 ug/ml PHA. Five x 105 cultured human U-937 cells per tube were washed and pre incubated 5 minutes with 20 ^l of 300 ^g/ml human IgG (to reduce non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-human CD155/Biotin at concentration of 10 ^g/ml. They were then washed twice and incubated with 2o reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.48 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD155-muIg. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

50 mM Sodium Phosphate, pH 7.5, 150 mM NaCl, 100 mM KCl, 0.04% Sodium Azide, 5% Glycerol, 0.2% BSA

Store at 4°C, avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P15151 NCBI: NM_006505 NP_006496.4