Target
Human PRKD1 / PKC Mu
Synonyms
PRKD1 | PKCM | Pkcmu | Protein kinase C, mu | Protein kinase D | NPKC-mu | PRKCM | Protein kinase C mu | Protein kinase D1 | NPKC-D1 | PKC-MU | PKD | Protein kinase C mu type
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-QKSPSESFIGREKR, from the internal region of the protein sequence according to NP_002733.2.
Applications
- IHC
- Western blot (0.1 - 0.3 µg/ml)
- Immunoprecipitation
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommended concentration 0.1-0.3 ug/ml IHC: This antibody has been confirmed in human pancreatic adenocarcinoma (PDA) according to Dappler et al, Sci Rep. 2016 Sep 21;6:33758 PMID 27649783. Immunoprecipitation: PKD1 was immunoprecipitated from Hek293T cells (as above). Immunoprecipitates were analyzed by Western Blot probed with the same antibody.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee