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Antibodies > PRKCA / PKC-Alpha > LS-C810064
 
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Catalog Number Size Price
LS-C810064-100 100 µl (1 mg/ml) $379 
LS-C810064-200 200 µl (1 mg/ml) $421 
PRKCA / PKC-Alpha Antibody - 1/100 staining human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PRKCA / PKC-Alpha Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCA / PKC-Alpha Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCA / PKC-Alpha Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PRKCA / PKC-Alpha Antibody - Western blot analysis of PKC-pan phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCA / PKC-Alpha Antibody - Western blot analysis of Phospho-PKC-pan (Thr497) expression in various lysates
PRKCA / PKC-Alpha Antibody - 1/100 staining human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PRKCA / PKC-Alpha Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCA / PKC-Alpha Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCA / PKC-Alpha Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.
PRKCA / PKC-Alpha Antibody - Western blot analysis of PKC-pan phosphorylation expression in NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCA / PKC-Alpha Antibody - Western blot analysis of Phospho-PKC-pan (Thr497) expression in various lysates
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Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Thr497, IHC, IF, WB) LS‑C810064

Polyclonal Rabbit anti‑Human PRKCA / PKC‑Alpha Antibody (phospho‑Thr497, IHC, IF, WB) LS‑C810064

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Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C810064-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKCA / PKC-Alpha Rabbit anti-Human Polyclonal (pThr497) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Alpha antibody LS-C810064 is an unconjugated rabbit polyclonal antibody to PKC-Alpha (PRKCA) (pThr497) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PRKCA / PKC-Alpha
Synonyms
PRKCA | Aging-associated gene 6 | AAG6 | Alpha PKC | PKC-A | Pkcalpha | Protein kinase C alpha type | PKC Alpha | PKC-alpha | PKCA | Protein kinase c alpha | Protein kinase C, alpha
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PKC-pan around the phosphorylation site of Threonine 497.
Epitope
pThr497
Specificity
Phospho-PKC-pan (Thr497) Antibody detects endogenous levels of PKC-pan only when phosphorylated at Threonine 497.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCA / PKC-Alpha
P17252 NM_002737 NP_002728.1

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PRKCA / PKC-Alpha Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: IHC, IHC - Paraffin, Western blot
See all PRKCA / PKC-Alpha Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States

Tel: (800) 227-6666 / (650) 697-3600

To place an order:
customerservice@vectorlabs.com

For questions about orders:
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